Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Table 2 MGMT methylation frequencies and methylation level in methylated glioma samples

From: MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

Group qMSP results Pyrosequencing results
   Median and IQR   Median and IQR
   calculated from PMR   calculated from
  Methylated samples MGMT
assay
MGMT_1
assay
Methylated samples mean CpG methylation
Low-grade gliomas 9/35
(25.7%)
3.30
(0.78-19.66)
0.50
(0.29-2.57)
34/35
(97.1%)
16.98
(10.97-38.29)
Astrocytoma
WHO grade II
2/10
(20.0%)
1.19
(0.85-1.54)
0.30
(0.29-0.30)
10/10 (100.0%) 12.56
(7.24-16.68)
Oligodendroglioma
WHO grade II
1/6
(16.7%)
0.78
-
0.87
-
5/6
(83.3%)
29.51
(24.23-33.17)
Oligoastrocytoma
WHO grade II
6/17
(35.3%)
11.72
(3.42-24.16)
1.54
(0.32-7.83)
17/17 (100.0%) 24.90
(14.41-47.52)
Low-grade neuroepithelial tumours
(not otherwise specified)
0/2
(0.0%)
- - 2/2
(100.0%)
6.83
(5.33-8.32)
High-grade gliomas 37/99
(37.4%)
9.18
(4.39-17.81)
5.59
(1.89-12.37)
54/99
(54.5%)
47.14
(18.50-62.87)
Anaplastic astrocytoma
WHO grade III
1/4
(25.0%)
24.19
-
22.35
-
2/4
(50.0%)
55.82
(52.95-58.70)
Anaplastic oligodendroglioma
WHO grade III
6/6
(100.0%)
19.30
(7.71-54.53)
11.61
(6.68-16.09)
6/6
(100.0%)
70.85
(52.98-82.52)
Anaplastic oligoastrocytoma
WHO grade III
1/3
(33.3%)
17.81
-
0.90
-
2/3
(66.7%)
52.99
(48.03-57.94)
Glioblastoma
WHO grade IV
29/86
(33.7%)
7.98
(4.00-14.55)
5.39
(1.89-11.37)
44/86
(51.2%)
43.31
(12.05-61.04)
   Primary glioblastoma 27/80
(33.8%)
8.67
(3.85-15.33)
5.59
(1.80-11.48)
40/80
(50.0%)
40.68
(11.12-59.05)
Included in survival analysis a 19/58
(32.8%)
5.53
(2.58-10.42)
3.22
(1.14-10.26)
29/58
(50.0%)
34.88
(10.64-48.73)
   Secondary glioblastoma 2/6
(33.3%)
5.76
(5.18-6.34)
2.75
(2.41-3.08)
4/6
(66.7%)
55.26
(42.67-63.93)
All glioma samples 46/134
(34.3%)
8.33
(3.40-18.55)
4.36
(0.88-11.54)
88/134 (65.7%) 34.03
(12.42-54.83)
  1. Abbreviations: PMR, Percentage of methylated reference, qMSP, quantitative methylation specific PCR, IQR, interquartile range
  2. a)Primary glioblastoma patients treated with standard radiation and at least concomitant temozolomide