MGMT promoter methylation in gliomas-assessment by pyrosequencing and quantitative methylation-specific PCR

Table 1 Primers and probes used for quantitative methylation-specific polymerase chain reaction (qMSP)

Assay	Sequence
MGMT qMSP ^a
Forward primer	GCGTTTCGACGTTCGTAGGT
Reverse primer	CACTCTTCCGAAAACGAAACG
Probe	6FAM-AAACGATACGCACCGCGA-MGB
MGMT_1 qMSP ^b
Forward primer	CGAATATACTAAAACAACCCGCG
Reverse primer	TTTTTTCGGGAGCGAGGC
Probe	6FAM-CGCGATACGCACCGTTTACG-MGB
ALU qMSP ^c
Forward primer	GGTTAGGTATAGTGGTTTATATTTGTAATTTTAGTA
Reverse primer	ATTAACTAAACTAATCTTAAACTCCTAACCTCA
Probe	6FAM-CCTACCTTAACCTCCC-MGB

Abbreviations: qMSP, quantitative methylation-specific polymerase chain reaction; MGB, minor groove binder
a) Modified after Rivera et al., Neuro Oncol. (2010)
b) Modified after Hoque et al., J. Natl. Cancer Inst. (2006)
c) Weisenberger et al., Nucleic Acids Res. (2005)

ISSN: 1479-5876