NOD/scid IL-2Rgnull mice: a preclinical model system to evaluate human dendritic cell-based vaccine strategies in vivo

Table 1 Composition of cocktails used for DC maturation

Cocktail	Inflammatory cytokines/interferons	Other additives	TLR-ligands
4C	TNF-α, IL-1β, IL-6	PGE₂
5C + R848	TNF-α, IL-1β, IFN-γ	PGE₂	poly(I:C), R848

The following concentrations were used in the maturation cocktails:
4 C: TNF-α (10 ng/ml); IL-1β (10 ng/ml); PGE₂ (1000 ng/ml); IL-6 (15 ng/ml)
5 C + R848: TNF-α (10 ng/ml); IL-1β (10 ng/ml); PGE₂ (250 ng/ml); IFN-γ (5000 U/ml); poly (I:C) (20 ng/ml); R848 (1 μg/ml)

ISSN: 1479-5876