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Table 1 Composition of cocktails used for DC maturation

From: NOD/scid IL-2Rgnull mice: a preclinical model system to evaluate human dendritic cell-based vaccine strategies in vivo

Cocktail Inflammatory cytokines/interferons Other additives TLR-ligands
4C TNF-α, IL-1β, IL-6 PGE2  
5C + R848 TNF-α, IL-1β, IFN-γ PGE2 poly(I:C), R848
  1. The following concentrations were used in the maturation cocktails:
  2. 4 C: TNF-α (10 ng/ml); IL-1β (10 ng/ml); PGE2 (1000 ng/ml); IL-6 (15 ng/ml)
  3. 5 C + R848: TNF-α (10 ng/ml); IL-1β (10 ng/ml); PGE2 (250 ng/ml); IFN-γ (5000 U/ml); poly (I:C) (20 ng/ml); R848 (1 μg/ml)