Figure 3

HER2-ECD specific antibody response. Serum from patients receiving dHER2-ASCI + lapatinib was analyzed for HER2-ECD specific antibodies by ELISA. Plates were coated with HER2-ECD protein and incubated with serial dilutions of patient sera (1:25- 1:6400), along with negative control serum and Herceptin as a positive control. Individual graphs of the HER2-ECD titer over time is represented for each patient (indicated by study number) with arrows indicating final dHER2ASCI injection. Although all patients had received trastuzumab at some point in the past, nine patients had received trastuzumab with their immediately prior systemic treatment regimen (range 21 to 37 days), while three had received the trastuzumab during a more distant systemic treatment reginmen. The numbers of days between receiving trastuzumab and starting study drug is noted on each graph as applicable. A * indicates samples taken once trastuzumab was restarted by the patient