Figure 5

Ad-IFNγ inhibited the growth of human NPC xenografts. When CNE-2 or C666-1 xenografts reached 4-5 mm diameter, mice were randomly assigned (7~8 mice per group) and treated by weekly intratumoral injection of 100 μL of PBS, Ad-LacZ or Ad-IFNγ (in 100 μL of PBS) or by daily intratumoral injection of 100 μL of normal saline (NS) or rhIFN-γ protein (in 100 μL of NS), respectively. (A, B) Tumor growth curves. Tumor size was measured every 4 days for CNE-2 xenografts (A) or 5 days for C666-1 (B), and the volume (V) was calculated according to the following formula: V = 0.52 × length × width²[18, 19]. (C, D) Tumor weights. After 3 weeks of treatment, mice with CNE-2 (C) or C666-1 (D) xenografts were sacrificed and tumor tissues were resected followed by weighing. (* p < 0.05, ** p < 0.01 compared with corresponding media).