Figure 2

Ad-IFNγ inhibited the proliferation of NPC cells by inducing G1 phase arrest in vitro . (A) Cell viability assay. CNE-2 or C666-1 cells were infected with Ad-IFNγ at different MOIs for 72 h, followed by MTT assay for cell viability. (B) Cell cycle distribution. CNE-2 or C666-1 cells were infected with Ad-IFNγ at 50 MOIs for 48 h, followed by flow cytometry analysis for DNA content and cell cycle distribution as described in “Materials and methods”. ^* p < 0.05, ^** p < 0.01 while compared with corresponding cells infected with Ad-LacZ, N=3.