Figure 1

Ad-IFNγ effectively expressed human IFN-γ in NPC cells. (A) Dose-dependent IFN-γ expression at mRNA level. After CNE-2 cells were infected with Ad-IFNγ at different multiplicities of infection (MOIs) (20 MOIs of Ad-LacZ as negative control) for 24 hours, human IFNγ mRNA levels were detected by RT-PCR in CNE-2 cells (IFNγ 473 bp; human β-actin as internal control, 334 bp). (B) Time course of IFN-γ expression at mRNA level. IFNγ mRNA levels were detected by RT-PCR in CNE-2 cells infected with 50 MOIs of Ad-IFNγ for 8, 24, 48 or 72 hours, CNE-2 cells infected with 50 MOIs of Ad-LacZ for 48 hours were used as negative control. (C) IFN-γ expression at protein levels in NPC cells. CNE-1 (left), CNE-2 (middle) or C666-1 (right) cells were infected with Ad-IFNγ at indicated MOIs, the culture supernatants were then sampled at different time points, and IFN-γ concentrations in the supernatants were detected by ELISA (Boster, Wuhan, China). IFN-γ expression efficiency was displayed as ng/10⁶ cells.