Figure 4

The analysis of mitochondrial function of C33a and SiHa cells. A, a: ROS generation was quantified by the fluorescence of H₂DCFDA for 30 min by fluorescence microscopy; b: Intracellular Ca²⁺ levels were monitored using the fluorescence probe fluo-4 AM; c: The relative Δψm values were measured by fluorescence of JC-1(590 nm: 527 nm). These data are representative of three separate experiments. *p < 0.05, **p < 0.01 versus p53 (-), gC1qR vector (+) and empty vector (-) group; ^Δp < 0.05, ^#p > 0.05 versus p53 (-), gC1qR vector (-) and empty vector (+) group; ^★★p < 0.01 versus p53 wt (+), gC1qR vector (-) and empty vector (+) group; ^▲p > 0.05 versus p53 mt (+), gC1qR vector (-) and empty vector (+) group. B, a: ROS generation was quantified by the fluorescence of H₂DCFDA for 30 min by fluorescence microscopy; b: Intracellular Ca^2± levels were monitored using the fluorescence probe fluo-4 AM; c: The relative Δψm values were measured by fluorescence of JC-1(590 nm: 527 nm). These data are representative of three separate experiments. **p < 0.01, ^#p > 0.05 versus gC1qR vector (+) group; ^▲p >0.05 versus empty vector (+) group; ^★★p <0.01, ^★★★p < 0.001 versus empty vector (+) group; ^Δp >0.05 versus empty vector (+) and p53 siRNA (+) group.