Figure 7

Combined treatment with NBD peptide and TMZ is more effective than TMZ alone in inducing senescence in M10 cells. (A) M10 cells were left untreated or exposed to 50 μM NBD peptide. After 24 h of culture, the cells were incubated with 50 μM TMZ+10 μM BG or with BG alone, and monitored 96 h later for the percentage of SA-β-Gal positive cells. Each value represents the arithmetic mean of three independent experiments. Bar, SEM. p values were calculated according to Student’s t test analysis. **p<0.01, TMZ+BG-treated cells versus BG-treated cells; *p<0.05, NBD peptide+BG-treated cells versus BG-treated cells; ^††p<0.01, NBD peptide+TMZ+BG-treated cells versus BG-treated cells; ^§§p<0.01 NBD peptide+TMZ+BG-treated cells versus TMZ+BG-treated cells and versus NBD+BG-treated cells; ^^p<0.01, TMZ+BG-treated cells versus NBD peptide+BG cells. (B) M10 cells were treated with NBD peptide and TMZ+BG as described in (A). After 7 days of culture, the cells were stained with DAPI to visualize SAHF formation.