Figure 3

TMZ promotes NF-κB2/p52 generation and nuclear translocation in M10 cells. (A) The cells were cultured in the presence of 50 μM TMZ+10 μM BG, or BG alone for the indicated time. Total cell extracts were then prepared and analyzed for NF-κB2/p52 expression by immunoblotting (see legend of Figure 2). The results are representative of two independent experiments. (B) The cells were cultured as described in (A) for 72 h. Nuclear extracts were then prepared and analyzed for NF-κB2/p52 content as described in (A) using anti-lamin A/C mAb for equal loading control. The results are representative of two independent experiments.