Impact of apoptotic adipose-derived mesenchymal stem cells on attenuating organ damage and reducing mortality in Rat sepsis syndrome induced by cecal puncture and ligation

Table 1 Flow cytometric analysis of H-ADMSC and A-ADMSC surface markers following Day-14 cell culture

Stem cell surface markers	H-ADMSC	A-ADMSC*	p-value
CD31+	4.4 ± 1.9	3.1 ± 2.2	0.871
CD34+	12.6 ± 5.6	11.2 ± 5.9	0.729
VEGF+	29.2 ± 8.9	15.9 ± 5.7	0.027
CD45+	13.7 ± 4.3	6.0 ± 3.3	0.022
C-kit+	14.3 ± 4.9	8.9 ± 2.8	0.028
Sca-1+	1.9 ± 0.7	1.6 ± 0.9	0.454
CD133+	8.6 ± 4.2	5.7 ± 3.4	0.312
CD90+	92.4 ± 3.7	81.4 ± 2.9	0.029
CD271+	28.3 ± 4.2	10.1 ± 2.9	0.004
Early apoptosis (annexin V+/PI-)	1.03 ± 1.17	10.48 ± 3.67	<0.0001
Late apoptosis (annexin V+/PI+)	4.27 ± 0.59	18.18 ± 4.75	<0.0001

Data are expressed as %.
n = 8 in each experimental study.
H = healthy; A = apoptosis; ADMSC = adipose-derived mesenchymal stem cell; VEGF = vascular endothelial growth factor.
* An additional 96 hour serum-deprived cell culture was performed for A-ADMSCs.

ISSN: 1479-5876