Figure 4

Transcript levels of acta 1and slc38a2 (Snat 2) in confluent L6 cells refed various groups of amino acids . L6 cells were cultured in the presence of low AA (0.28 mM) or high AA (9 mM) concentrations of all amino acids, or in the presence of high concentrations of various groups of amino acids (BCAA, aromatics, gln) in addition to background concentration of all amino acids in DMEM (0.14 mM) as described in Material and Methods. All cells were cultured in medium with decreased amounts of all amino acids (0.14 mM) for an initial period of 24 hours. Media were changed and cells were cultured in the presence of either low AA (0.28 mM), high AA (9 mM) or group amino acids for further 18 hours. (* p< 0.05 vs. refed low). RFBCAA = refed by BCAA (2.5 mM), RFArom = refed by aromatic amino acids (1mM), RFGlu = refed by Gln, Lys, Arg, Thr, His (5.3 mM).