Figure 1
Electrophoretic separation of cytoskeletal proteins in L6 cells by stepwise purification . Cytoskeletal proteins were extracted using Calbiochems sub-cellular proteome extraction kit and separated by electrophoresis as described in Material and Methods. Lane 1–2 are autoradiograms of 35S-methionine labeled cells from low AA (lane 1) and high AA refed (lane 2) cells. Lane 3–4 are the same samples stained by Coomassie brilliant blue. Lane 5 is purified rabbit muscle actin (Sigma-Aldrich A-2522). Lane 6 is purified rabbit muscle myosin (Sigma-Aldrich M-1636). It has been demonstrated that L6 cells contain myosin [44].