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Table 3 The levels of cytokines in whole blood culture supernatants in MS patients

From: A gender-related action of IFNbeta-therapy was found in multiple sclerosis

 

Treated Patients

Untreated Patients

Cytokines(pg/ml)

Basic condition

Basic condition

 

Men

Women

Men

Women

IL10

2.13 ± 0.4

1.8 ± 0.2

1.5 ± 0.1

1.8 ± 0.2

IFNγ

16.1 ± 5

11.4 ± 3.2

14 ± 2.3

19.8 ± 3.6

IL6

232 ± 227a

10 ± 9.8a

35.8 ± 11.5

12.0 ± 17.5

IL2

10.8 ± 1

8.7 ± 0.9

8.7 ± 3.5

9.4 ± 1.5

IL4

1.1 ± 0.2

1.1 ± 0.1

0.7 ± 0.1

0.7 ± 0.1

IL12p70

9.2 ± 0.9

9.5 ± 0.8

11.2 ± 1.2

8.4 ± 0.7

IL12p40

25.2 ± 2.6

21.6 ± 3

20.1 ± 6.5

20.7 ± 5.1

TGFβ

7324 ± 833

5903 ± 458

10721 ± 5822

5758 ± 386

 

PHA

PHA

 

Men

Women

Men

Women

IL10

59.7 ± 24.9

23.7 ± 11.8

78 ± 17.4

49.4 ± 30.2

IFNγ

1149.2 ± 570

740 ± 112.9b

2238 ± 904

2102 ± 658b

IL6

759 ± 359

627 ± 109.1

673 ± 427

648.1 ± 153.8

IL2

18.3 ± 5.9

13.9 ± 7.4

20.6 ± 8

21.3 ± 4.1

IL4

9.7 ± 5.3

3.6 ± 1.3

3.6 ± 1.1

1.2 ± 1.5

IL12p70

13.4 ± 2.5

9.9 ± 0.6

9.1 ± 1.5

9.3 ± 0.9

IL12p40

196 ± 105

101.7 ± 15.4

186 ± 82

86.2 ± 15.4

TGFβ

7644 ± 906

6953 ± 583

16029 ± 8457

8612 ± 4630.1

 

LPS

LPS

 

Men

Women

Men

Women

IL10

144 ± 28.2

215 ± 54.7

181 ± 50

195.5 ± 48.6

IFNγ

135 ± 37.9

71.2 ± 139.4

1131 ± 809.3

263 ± 88.5

IL6

989 ± 89

635.9 ± 112.5

795 ± 173

813.2 ± 123.7

IL2

15.6 ± 3.7c

11 ± 4.4

7.6 ± 1.5c

12 ± 1.6

IL4

1.54 ± 0.5

1.1 ± 0.1d

0.7 ± 0.1

0.7 ± 0.06d

IL12p70

11.5 ± 1.9

8.9 ± 0.8

9.7 ± 0.9

9.1 ± 0.7

IL12p40

416 ± 309

354.4 ± 69.5

1203 ± 664

284.2 ± 87.2

TGFβ

7582 ± 832

7524.6 ± 3492.7e

12669 ± 6570

16984 ± 4854.8e

  1. Levels were determined by whole blood assays in resting conditions (Basic conditions) and after stimulation with PHA (to principally study the activated T cell network contribution) and LPS (to evaluate the influence of activated antigen presenting cells). The level values are expressed as mean ± SD and statistical differences between groups were assessed using the Mann–Whitney U-test or Student’s t-test as appropriate: aa p = 0.027; bb p = 0.023; cc p = 0.039; dd p = 0.002; ee p = 0.031.