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Table 2 Partially reversal effect after ATP7A's silence by siRNA for 80 h in the DDP-resitant subline A549/DDP

From: Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC)

cell lines IC50 (mean ± SD, uM) areversal effect(%) P
A549/DDP 29.33 ± 0.20   
A549/DDP Control SiRNA 29.51 ± 0.17   > 0.05
A549/DDP ATP7A SiRNA 20.77 ± 0.28 29.62 < 0.001
  1. ATP7A siRNA group is A549/DDP cells treated with ATP7A siRNA sequences at the final 100 nM concentration for 8 hrs and then added DDP at various concentrations for 72 hrs. Control siRNA group is A549/DDP cells treated with nonsilencing siRNA sequences at the final 100 nM concentration for 8 hrs and then added DDP at various concentrations for 72 hrs. a, reversal effect was determined by the percentage of the IC50 difference between Control siRNA group and ATP7A siRNA group to the IC50 of Control SiRNA groups. Data represents the mean ± SD of at least three independent experiments performed with 8 wells at each drug concentration tested. P < 0.05 was considered as a significant change in drug sensitivity (Student's t-test). SD, standard deviation; DDP, cisplatin