Figure 3

Increasing DDP induced apoptosis after ATP7A's silence for 80 h by siRNA in A549/DDP cells. Apoptotic or necrotic cell death was tested by flow cytometric analysis. Control siRNA, added 100 nM nonsilencing siRNA sequences for 8 h and then added DDP for 72 h; ATP7A siRNA, added 100 nM SiRNA targeting ATP7A for 8 h and then added DDP for 72 h. Columns, mean of three independent experiments; bars, SD.