Table 1 PCR primer sequences, G6PD gene fragments amplified and PCR product sizes
From: Five novel glucose-6-phosphate dehydrogenase deficiency haplotypes correlating with disease severity
Frag-ment | Exon(s) | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) | PCR product size |
|---|---|---|---|---|
1 | 1,2 | CAATAGGGCCGGCTTGAC | TGCAACAATTAGTTGGAAAAGC | 1376bp |
2 | 3,4,5 | TGTCCCCAGCCACTTCTAAC | CTCATAGAGTGGTGGGAGCA | 1125bp |
3 | 6,7 | AGGGGTTCAAGGGGGTAAC | TGCAGGGTGACTGGCTCT | 563bp |
4 | 8 | ACAGGGCGGGGAGCTAAG | GTGCCTCGTCACAGATGG | 202bp |
5 | 9,10 | CCTGAGGGCTGCACATCT | GTGTCTTGCTGATGCCACTG | 741bp |
6 | 11,12,13 | TGGCATCAGCAAGACACTCT | GACAAGGAAGTGGGTCCTCA | 1151bp |