Table 1 Overview of the technical specifications of the ELISA’s applied in the experiment with CIA rats
Assay name | Target | Specificity of assay | Detection range (ng/ml) | Intra-assay variation (%) | Inter-assay variation (%) |
|---|---|---|---|---|---|
C1M[20] | Degradation of type I collagen | Neo-epitope generated by MMP-2, 9, and 13 | 0.83-500 | 6.7 | 10.1 |
C2M[21] | Degradation of type II collagen | Neo-epitope generated by several MMP | 0.09-2 | 3.5 | 7.3 |
C3M[22] | Degradation of type III collagen | Neo-epitope generated by MMP-9 | 0.86-50 | 2.7 | 3.4 |
P1NP[23] | Formation of type I collagen | N-terminal pro-peptide | 0-1000 | n.a. | n.a. |
P2NP (unpublished) | Formation of type II collagen | N-terminal pro-peptide | 0-1000 | n.a. | n.a. |
P3NP (unpublished) | Formation of type III collagen | N-terminal pro-peptide | 0.149-76.3 | 4.1 | 11.0 |