Figure 1
From: Gene silencing of IL-12 in dendritic cells inhibits autoimmune arthritis
Gene silencing of IL-12 shRNA on DC. (A) Gene silencing efficacy of IL-12 shRNA was determined by RT-PCR. DC were cultured for 4 days as described in the methods. On day 5, 106 DC were transfected with 2 μg of IL-12 shRNA using GenePORTER reagent. One day after transfection, DC were harvested and mRNA detected. (B) Gene silencing efficacy of IL-12 shRNA was determined by flow cytometry. DC were transfected as above and collected 48 h after transfection. DC were intracellular stained with FITC labeled anti-IL-12 antibody and followed by flow cytometry analysis. The data presented one of three independent experiments.