Real time PCR of miR-200c in blood samples. The graph depicts the increasing relative expression levels for the mean blood expression levels of miR-200c (Kruskal-Wallis test, p =0.018) from controls (n = 15) and gastric cancer samples (n = 52). Significant differences were observed between the blood expression levels of miR-200c in each TNM stage subgroup and the control group (Bonferroni post hoc test: stage I-III vs control, *p = 0.018; stage IV vs control, **p < 0.001). MiR-200c was measured in triplicate using qRT-PCR and normalised to U6 snRNA and 5S rRNA. The horizontal bar denotes the mean value for each group.