Real-time PCR of the miR-200 family in control blood and gastric cancer cell lines. The raw quantification cycle (Cq) data for the miR-200 cluster in the control blood samples (n = 19) and gastric cancer cell lines (OE-19 and MKN-45) are depicted. In the blood, the mean Cq was lower for miR-141 (Cq = 28) compared with those for miR-200a (Cq = 35), 200b (Cq = 35) and 200c (Cq = 35; ANOVA, p < 0.001; Bonferroni post hoc test, p < 0.001). In the GC cell lines, the mean Cqs were 15.3, 16.7, 17.7 and 16.1 for miR-141, 200a, 200b and 200c, respectively, without significant differences (ANOVA, p = 0.133). The red boxes indicate control blood samples, while the light-blue boxes indicate the gastric cancer cell lines.