Table 1 Sequences of primers used for qMSP and qRT-PCR assays
From: Whole genome methylation profiles as independent markers of survival in stage IIIC melanoma patients
qMSP | ||
|---|---|---|
GENE | Primer sequence1 | Amplicon size (bp)2 |
SLC6A11 | MF: TGTTTAGGGTTGGGAAGAAGTTAC | 128 |
MR: ATCGCAATAAACTAAAAAACCTACG | ||
UF: TGTTTAGGGTTGGGAAGAAGTTAT | 133 | |
UR: AATAAATCACAATAAACTAAAAAACCTACA | ||
TUB | MF: TGGTTGTTAGTTTGATTGTTGTTAC3 | 96 |
MR: AAAACCTATTAAAATTCCCTATATTCG | ||
UF: GTGGTTGTTAGTTTGATTGTTGTTAT | 97 | |
UR: CTAAAACCTATTAAAATTCCCTATATTCA | ||
ALOX12B | MF: TTCTCTTACCTACCTTAAACCTTCG | 152 |
MR: TGAGATGGAGTTTCGTGTTTTC | ||
UF: TTCTCTTACCTACCTTAAACCTTCA | 154 | |
UR: AGTGAGATGGAGTTTTGTGTTTTT | ||
WNT10B | MF: TGGGGTGTATAGGTAAAGGTAAATC | 91 |
MR: GAAAATAAATCAAACGAAAACACG | ||
UF: TGGGGTGTATAGGTAAAGGTAAATT | 93 | |
UR: TCAAAAATAAATCAAACAAAAACACA | ||
qRT-PCR | ||
GENE | Primer sequence | Amplicon size (bp) |
ALOX12B | F: ACCCGAGGGCAAGATGAT | 74 |
R: GCAGGAAGATGGGGCAAT | ||
SLC6A11 | F: AGGGGGTACCCATTGCTG | 65 |
R: CTTGGGGTACGCAATAAAGG | ||
TUB | F: TCCGACTGGATTCCCTACAG | 109 |
R: GGCGCTTCTTCTTCTGCTT | ||
WNT10B | F: GCGAATCCACAACAACAGG | 107 |
R: TCCAGCATGTCTTGAACTGG | ||