Table 4 Association of CDKN2A methylation and clinico-pathological features stratified by microsatellite instability (MSI) status
Feature | CDKN2A in MSS/MSI-L (n;%) | P-value | CDKN2A in MSI-H (n;%) | P-value | |||
|---|---|---|---|---|---|---|---|
Negative | Positive | Negative | Positive | ||||
(n = 272; 81.4%) | (n = 62; 18.6%) | (n = 42; 68.9%) | (n = 19; 31.2%) | ||||
Age | Mean (range) | 69.5 (40–95) | 69.9 (44–88) | 0.788 | 68.4 (45–87) | 68.2 (50–89) | 0.9897 |
Tumor size | Mean (range) | 48.0 (5–130) | 49.8 (4–105) | 0.523 | 54.9 (19–160) | 62.8 (15–170) | 0.3737 |
Tumor location | Left-sided | 193 (71.2) | 34 (54.8) | 0.0125 | 24 (57.1) | 4 (22.2) | 0.013 |
Right-sided | 78 (28.8) | 28 (45.2) | 18 (42.9) | 14 (77.8) | |||
Gender | Female | 149 (54.8) | 34 (54.8) | 0.9932 | 20 (47.6) | 10 (52.6) | 0.7169 |
Male | 123 (45.2) | 28 (45.2) | 22 (52.4) | 9 (47.4) | |||
Histological subtype | Non-mucinous | 253 (93.0) | 54 (87.1) | 0.1142 | 39 (92.9) | 16 (84.2) | 0.2863 |
Mucinous | 16 (5.9) | 8 (12.9) | 3 (7.1) | 2 (10.5) | |||
Tumor grade | G1-2 | 261 (98.1) | 54 (87.1) | <0.0001 | 37 (88.1) | 14 (73.7) | 0.2608 |
G3 | 5 (1.9) | 8 (12.9) | 5 (11.9) | 5 (26.3) | |||
pT classification | pT1-2 | 59 (22.1) | 13 (21.0) | 0.8354 | 12 (28.6) | 0 (0.0) | 0.0093 |
pT3-4 | 207 (77.8) | 49 (79.0) | 30 (71.4) | 19 (100.0) | |||
pN classification | pN0 | 140 (53.4) | 23 (37.1) | 0.0207 | 26 (66.7) | 10 (52.6) | 0.3012 |
pN1-2 | 122 (46.6) | 39 (62.9) | 13 (33.3) | 9 (47.4) | |||
Vascular invasion | Absent | 186 (69.9) | 37 (59.7) | 0.1193 | 35 (83.3) | 11 (57.9) | 0.0326 |
Present | 80 (30.1) | 25 (40.3) | 7 (16.7) | 8 (42.1) | |||
Invasive margin | Pushing | 69 (26.0) | 12 (19.7) | 0.2996 | 26 (61.9) | 6 (31.6) | 0.0281 |
Infiltrating | 196 (74.0) | 49 (80.3) | 16 (38.1) | 13 (68.4) | |||
Peritumoral lymphocytic inflammation | Absent | 211 (79.3) | 51 (82.3) | 0.6037 | 27 (64.3) | 13 (68.4) | 0.7529 |
Present | 55 (20.7) | 11 (17.7) | 15 (35.7) | 6 (31.6) | |||
KRAS (codon 12/13) | Wild-type | 185 (69.0) | 41 (68.3) | 0.9161 | 26 (65.0) | 14 (93.3) | 0.0356 |
Mutation | 83 (31.0) | 19 (31.7) | 14 (35.0) | 1 (6.7) | |||
BRAF (codon V600E) | Wild-type | 245 (96.8) | 42 (75.0) | <0.0001 | 30 (79.0) | 8 (44.4) | 0.0098 |
Mutation | 8 (3.2) | 14 (25.0) | 8 (21.0) | 10 (55.6) | |||