Figure 1

Stress fiber staining in isolated fibroblasts. Stress fibers were visualized in centrally (A and B) and distally (C and D) derived fibroblasts from control subjects (A and C) and COPD patients (B and D) by staining with phalloidin that binds to F-actin. Contractile cells have parallel fibers attached to their lamellipodia as is indicated by arrowheads in (D) Other cell types do not have this feature to the same extent. Scale bars represent 50 μm.