Laser Doppler scanning of hind limb blood flow on day 21 after CLI induction (n = 8). (A) (simultaneous scanning of both hind limbs), (B) (isolated left hind limb scanning) and (C) (isolated right hind limb scanning) in normal rats. D) Normal blood flow in both hind limbs among five groups prior to the procedure. F to J) ratio of ischemic/normal blood flow (INBF) among five groups (F = control, G = CLI only, H = CLI + cilostazol, I = CLI + clopidogrel, J = CLI + combined therapy) by day 21 after CLI procedure. E)* vs. other groups with different symbols, p < 0.001. Statistical analysis by ANOVA followed by Bonferroni multiple comparison post hoc test. CLI = critical limb ischemia; Cil = cilostazol; Clo = clopidogrel.