Figure 4

Western blot analysis in PANC-1 cells of the cell cycle proteins Cdk4 and cyclin D1. (A) Samples were obtained from PANC-1 cells treated with 300 nM of P276-00 for 72 h or 70 nM gemcitabine for 24 h or the combination of gemcitabine for 24 h followed by P276-00 for 72 h. (B) Densitometric analysis of the Cdk4 and cyclin D1 bands using the software Image J.