The generation of antigen-specific CD8.+T cells in DPX-0907 vaccinated ovarian, prostate and breast cancer patients. Patient PBMC were stimulated with indicated peptides in vitro as described in Materials and Methods. Cells were stained with corresponding MHC-peptide pentamer reagents to detect CD8+ T cells with peptide-specific TCR repertoire. HIV-pentamer served as a negative control (patients pre-screened for being HIV negative) and CMV-specific pentamer was used on a known CMV-positive donor PBMC as internal positive control for the assay (data not shown). Samples from all study time points were run simultaneously in the assay for each patient. Data represented as percentage of live gated CD3+CD8+ cells that were positive for pentamer staining. The background staining on CD8 negative cells (0.02-0.06%) has been subtracted from the values shown and the values for control HIV-pentamer staining were in the range of 0.00 to 0.03% for all patients.