Figure 1

Identification of iNOS⁺cells in 4 T-1 tumor. A. Single-cell suspensions were prepared from established tumors. FACS analysis of tumor-resident heterogeneous myeloid cell populations identified four distinct subsets by the expression of the surface markers CD11b and Gr-1. Expression of iNOS coupled with F4/80 was evaluated within each gated population. The P1 subset was divided into two subfractions P1a and P1b based on iNOS expression B. Ly6G/C and FSC/SSC dot plots are shown for gated P1a, P1b and P4 cell subsets. A back gating strategy revealed distinct locations for these subsets on a dot plot graph based on their FSC and SSC profile. P1a (F4/80⁺iNOS⁺), P1b (F4/80⁺iNOS^-), and P4 (F4/80^-CD11b^hi) subsets were further evaluated based on Ly6G andLy6C expression. C. The isotype antibody staining (gray filled area) from single-cell suspensions for iNOS is shown.