Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice

Table 1 Primer Used for Real-Time PCR Amplification

Gene	GenBank accession number	Forward Primer (5 ´-3 ´)	PCR product size (bp)
		Reverse Primer (5 ´-3 ´)
IL-6	NM_012589.1	CCCTTCAGGAACAGCTATGAA	74
		ACAACATCAGTCCCAAGAAGG
MMP-9	NM_031055.1	CCTCTGCATGAAGACGACATAA	64
		GGTCAGGTTTAGAGCCACGA
Leptin	NM_008493.3	CAGGATCAATGACATTTCACACA	145
		GCTGGTGAGGACCTGTTGAT
IL-1β	NM_214055.1	CCTGGACCTTGGTTCTCTGA	61
		CCAGAGCTGGTGAGAGATTTG
Adiponectin	NM_009605.4	GGAGAGAAAGGAGATGCAGGT	109
		CTTTCCTGCCAGGGGTTC
PAI-1	NM_213910.1	GCCCTGGTGACTCATTTCA	65
		CTCTCACGTGTCCACTGCTC
IFN-γ	NM_008337.3	ATCTGGAGGAACTGGCAAAA	89
		TTCAAGACTTCAAAGAGTCTGAGGTA
ET-1	M64711.1	TGTCTACTTCTGCCACCTGGA	69
		CCTAGTCCATACGGGACGAC
AT-1R	NM_030985.4	CACCCGATCACCGATCAC	110
		CAGCCATTTTATACCAATCTCTCA
Bax	NM_017059.1	GTGAGCGGCTGCTTGTCT	107
		GACTCCAGCCACAAAGATGG
Caspase 3	NM_009810.2	TGAAGACATTTTGGAATTAATGGA	90
		TCACCATGGCTTAGAATCACA
Bcl-2	NM_016993.1	GGGATGCCTTTGTGGAACT	82
		CTGAGCAGCGTCTTCAGAGA
HO-1	NM_001004027	CATTCAGCTGTTTGAGGAGGT	64
		CGAGGGTCTCTGGTCCTTAGT
NQO-1	NM_001159613	CCGAGAAGACGTCCTTCAAT	96
		TGGCATACAGGTCCGACAC
α-actin	DQ452569	CTGGACTTCGAGCAGGAGAT	69
		GGCAGCTCGTAGCTCTTCAT

IL = interleukin; MMP = matrix metalloproteinase; PAI = lasminogen activator inhibitor; IFN = interferon; ET = endothelin; AT-1R = angiotensin II type 1 receptor; HO = heme oxygenase; NQO = NAD(P)H quinone oxidoreductase.

ISSN: 1479-5876