Figure 2

Bürker chamber and Fast Read 102® cell count method. The Bürker chamber has 9 large squares (1 mm² each), divided by double lines (0.05 mm apart) into 16 group squares. The double lines form small 0.0025 mm² squares. The Chamber depth is 0.1 mm. The cells were counted in each of the 4 large squares (identified by the triple line and shaded in the figure). At the end of the procedure the operators calculate the average of the 4 readings (from 4 large squares) and calculate the cell concentration as follows:. $\left[\frac{\text{Cell}}{ml}\right]=\left(\frac{\sum \text{Cells}\text{counted}in4\text{squares}}{4}\right)\times \text{dilution}\text{factor}\times {10}^4$. Fast Read 102® chamber, a plastic device with a slide divided into 10 chambers. Each chamber contains a grid with 10 squares, subdivided into 16 small squares. When the chamber was filled, the cells distributed in the 5 squares (black lines) were counted, taking into consideration, for each chamber, a size of 1 x 1 mm, a depth of 0.1 mm and a volume of 0.1 μl per square, the cell concentration (cells/ml) was determined by the formula: $\left[\frac{\text{Cell}}{ml}\right]=\left(\frac{\sum \text{cells}\text{counted}in5\text{squares}}{5}\right)\times \text{dilution}\text{factor}\times {10}^4$