Mice with approximately 100 mm3 B16 or CT26 tumors growing subcutaneously were treated by intratumoral administration of 5 × 108 pfu agents in the top column once a day for 2 consecutive days. Tumors were excised after 72 h and animals were re-inoculated with autologous tumor cells 2 weeks after surgery. Positive tumor formation was determined by at least minimal, palpable tumor 2 weeks from the time of tumor injection. Untreated animals as a positive control were injected and showed tumor formation. *represent statistical significance (P < 0.05, Adel55-cHSF1 vs. Adel55-HSP70 or Adel55-HSP90).