Figure 3

Kaplan–Meier survival analysis of mice after re-challenging with autologous tumor. C57B/6 or Balb/C mice with approximately 100 mm³ B16 (A, B, C) or CT26 tumors (D, E, F) growing subcutaneously were treated by intratumoral administration of 5 × 10⁸ pfu of PBS, Adel55, Adel55-cHSF1 or Adel55-HSF1i once a day for 2 consecutive days. Tumors were excised after 72 h. Animals were re-inoculated with autologous tumor cells subcutaneously 2 weeks after surgery and followed for survival. Criteria for removal of animals from consideration included weight loss > 12%, inability to ambulate, inability to feed, or loss of normal grooming. Data shown are from three independent experiments for both tumor models (n = 4-6 for all groups). (*) denotes statistical significance (Adel55-cHSF1 vs. PBS or Adel55 or Adel55-HSF1i).