Table 3 Associations of HER2 and TOP2A with ER, PgR and Ki67 status (assessed by IHC)
| Â | ER status | Â | PgR status | Â | Ki67 status | Â | |||
|---|---|---|---|---|---|---|---|---|---|
| Â | Negative | Positive | p | Negative | Positive | p | Low | High | p |
| Â | N (%) | N (%) | Â | N (%) | N (%) | Â | N (%) | N (%) | Â |
HER2 (CISH) | Â | Â | < 0.001 | Â | Â | 0.001 | Â | Â | 0.035 |
   Non-amplified | 47 (61.8) | 173 (83.2) |  | 63 (65.6) | 156 (83.4) |  | 42 (89.4) | 176 (75.2) |  |
   Amplified | 29 (38.2) | 35 (16.8) |  | 33 (34.4) | 31 (16.6) |  | 5 (10.6) | 58 (24.8) |  |
HER2 (mRNA) | Â | Â | < 0.001 | Â | Â | < 0.001 | Â | Â | 0.086 |
   Low (< 75th perc) | 38 (55.9) | 166 (82.6) |  | 53 (59.6) | 151 (84.4) |  | 40 (87.0) | 168 (74.0) |  |
   High (≥ 75th perc) | 30 (44.1) | 35 (17.4) |  | 36 (40.4) | 28 (15.6) |  | 6 (13.0) | 59 (26.0) |  |
HER2 (IHC) | Â | Â | 0.003 | Â | Â | 0.002 | Â | Â | 0.016 |
   0-1+ | 47 (58.8) | 163 (75.5) |  | 61 (58.7) | 148 (77.5) |  | 43 (87.8) | 163 (67.4) |  |
   2+ | 9 (11.3) | 25 (11.6) |  | 15 (14.4) | 19 (9.9) |  | 3 (6.1) | 31 (12.8) |  |
   3+ | 24 (30.0) | 28 (13.0) |  | 28 (26.9) | 24 (12.6) |  | 3 (6.1) | 48 (19.8) |  |
TOP2A (CISH) | Â | Â | 0.29 | Â | Â | 0.46 | Â | Â | 0.33 |
   Non-amplified | 64 (90.1) | 182 (93.8) |  | 83 (91.2) | 162 (93.6) |  | 41 (97.6) | 202 (91.8) |  |
   Amplified | 7 (9.9) | 12 (6.2) |  | 8 (8.6) | 11 (6.4) |  | 1 (2.4) | 18 (8.2) |  |
TOP2A (mRNA) | Â | Â | 0.99 | Â | Â | 0.20 | Â | Â | 0.003 |
   Low (< median) | 34 (50.0) | 100 (49.8) |  | 39 (43.8) | 94 (52.5) |  | 33 (71.7) | 107 (47.1) |  |
   High (≥ median) | 34 (50.0) | 101 (50.2) |  | 50 (56.2) | 85 (47.5) |  | 13 (28.3) | 120 (52.9) |  |
TopoIIa (IHC) | Â | Â | 0.39 | Â | Â | 0.99 | Â | Â | < 0.001 |
   Negative | 29 (40.3) | 69 (34.5) |  | 33 (35.9) | 64 (35.8) |  | 32 (78.0) | 66 (28.8) |  |
   Positive | 43 (59.7) | 131 (65.5) |  | 59 (64.1) | 115 (64.2) |  | 9 (22.0) | 163 (71.2) |  |