Figure 6
Time course of GrB and IFN-γ secretion in the ELISPOT assays and cytotoxicity in 51Cr-release assay. Human αFMP-CTL (7 day culture) were used as effector cells (5 × 103 cells per well). Target cells were C1R.A2 pulsed with FMP (5 × 104 cells per well). Effector and target cells were incubated for the specified times at 37°C. A table containing the number of spots per 105 cells is provided to better clarify the data. Background spots (CTL alone) were subtracted from the results. Data is an average from three experiments.