Figure 5
Effector cells secreting GrB and IFN-γ in the ELISPOT assays. Human αFMP-CTL (7 day culture) or cultures depleted of CD8+ cells were used as effector cells (5 × 103 cells per well). Target cells were C1R.A2 pulsed with FMP (5 × 104 cells per well). CD8+ cells were removed from effector cell cultures using anti-CD8 mAb and magnetic beads. Effector and target cells were incubated for 4 h at 37°C. Data is presented as spots per 105 cells ± SD and is representative of 3 experiments.