Figure 4
Assessment of the IFN-γ protein release kinetics by ex vivo peptide-stimulated PBMCs. Two donors, one CMV seropositive and one CMV seronegative, were exposed at various durations of time to both FluM158–66 and CMVpp65495–503 peptides and cell supernatants were analyzed for IFN-γ protein release (panel A and B). Panel C shows the protein release from both FluM158–66 and CMVpp65495–503 2-week sensitized PBMCs from the CMV seropositive donors either after an 18-hour peptide re-exposure or after no peptide re-exposure. Results represent the mean ± SEM of three independent experiments carried out in triplicate.