Fig. 5

CAV3 overexpression restored mitochondrial function partially relied on NDUFA10 in vivo and in vitro models. A Representative transmission electron microscope images of cardiac mitochondria and quantification analysis of mitochondrial cristae number and the proportion of mitochondria with disorganized cristae in different db/db mice groups (n = 7 mice). B Normalized ATP production levels in cardiomyocytes of db/db mice transfected with or without AAV-CAV3 or AAV-shNDUFA10 (n = 7 mice). C Flow cytometry and quantitative analysis of mitochondrial membrane potential by JC-1 in cardiomyocytes treated with HGHF and transfected with or without pc3.1-CAV3 or siNDUFA10 (n = 5). D Extracellular flux analysis of OCRs and respective quantitative analysis in cardiomyocytes treated with HGHF and transfected with or without pc3.1-CAV3 or siNDUFA10 (n = 3). E The activity of mitochondrial complex I in cardiomyocytes of db/db mice transfected with or without AAV-CAV3 or AAV-shNDUFA10 (n = 7 mice). F Representative images of DHE staining and quantitative analysis of ROS levels in the hearts of db/db mice transfected with or without AAV-CAV3 or AAV-shNDUFA10 (n = 7 mice). G Representative images of immunofluorescence staining of 8-oxoG and quantitative analyses in cardiomyocytes treated with HGHF and transfected with or without pc3.1-CAV3 or siNDUFA10 (8-oxoG fluorescence, red) (n = 5). H Representative Western blotting and quantitative analyses for SOD2, Bcl2, Bax and Cleaved caspase 3 protein levels in cardiomyocytes of db/db mice transfected with or without AAV-CAV3 or AAV-shNDUFA10 (n = 7 mice). I TUNEL assay by double staining with TUNEL (red) and DAPI (blue) detected apoptotic cells in the hearts and quantitative analysis of different db/db mice groups (n = 7 mice). Data are depicted as mean ± SD. *p < 0.05, **p < 0.01