Fig. 3

Overexpression of CAV3 prevented mitochondrial dysfunction in diabetic cardiomyocytes in vivo and in vitro models. A Representative transmission electron microscope images of cardiac mitochondria and quantification analysis of mitochondrial cristae number and the proportion of mitochondria with disorganized cristae in wt mice and db/db mice with or without CAV3 overexpression (n = 7 mice). B Normalized ATP production levels in cardiomyocytes of wt mice and db/db mice with or without CAV3 overexpression (n = 7 mice). C Flow cytometry analysis and quantification of mitochondrial membrane potential by JC-1 in cardiomyocytes treated with or without HGHF and with or without CAV3 overexpression. (n = 5). D Extracellular flux analysis of OCRs and respective quantitative analysis in cardiomyocytes treated with or without HGHF and with or without CAV3 overexpression (n = 3). E Representative images of DHE staining and quantitative analysis of ROS levels in the hearts of wt mice and db/db mice with or without CAV3 overexpression (n = 7 mice). F Representative images of immunofluorescence staining of 8-oxoG and quantitative analyses in cardiomyocytes treated with or without HGHF and with or without CAV3 overexpression (8-oxoG fluorescence, red) (n = 5). G Representative Western blotting and quantitative analyses for SOD2, Bcl2, Bax and Cleaved caspase 3 protein levels in cardiomyocytes of wt mice and db/db mice with or without CAV3 overexpression (n = 7 mice). H TUNEL assay by double staining with TUNEL (red) and DAPI (blue) detected apoptotic cells in mice hearts and quantitative analysis of wt mice and db/db mice with or without CAV3 overexpression (n = 7 mice). Data are depicted as mean ± SD. *p < 0.05, **p < 0.01