Fig. 3

Comparison of Pime-Seq and lrPCR-NGS. A flow diagram of the comparison study; B HF correlation of the 1677 concordant variants detected by Pime-Seq and lrPCR-based NGS in 45 human PBMC samples. The HF is indicated by the numbers on both the horizontal and vertical axes. Correlations and P values are based on Spearman’s correlation; C Evaluating the reliability of the 146 discordant variants by analyzing them in human mitochondrial genome databases; D Verification of the discordant result of m.9540T > C in sample 22MS0027 using pyrosequencing