Fig. 2

Venn diagram illustrating the coding genetic variant shared among patients exhibiting long-lasting disease control with TAS-102 (FA, CC, and NA represent patients' initials). The sole shared genetic variation (highlighted in red) is FGFR4 p.G388R. DNA extraction was performed from formalin-fixed and paraffin-embedded (FFPE) primary colorectal cancer tissue specimens. Libraries were prepared using the TruSigtTMOncology 500 kit, which assesses 523 cancer-relevant genes, and sequenced on an Illumina NovaSeq 6000 platform. Sequences were aligned to the human reference genome GRCh37 using the Burrows–Wheeler Aligner tool (for further details, refer to the Methods section)