Fig. 5

PLCB1 inhibitor U73122 suppress proliferation, induce cell cycle arrest, and activate apoptosis of leukemic cells in vitro. (A) Sample-wise proportion of cells in G1, S and G2M phase in total HSPCs. (B) Sample-wise proportion of cells in G1, S and G2M phase in CMP. (C) GSEA plots showing pathways of cell cycle G1/S phase transition enriched in CMP of MDS patients. (D) CCK-8 assay of cell viability in THP-1 cell line with different concentrations of U73122 for 24 and 48 h respectively. (E) Cell apoptosis analysis of THP-1 cells treated with 0µM, 5µM or 10µM U73122 for 24 h. (F) Cell cycle analysis of THP-1 cells treated with 0µM, 5µM or 10µM U73122 for 24 h. (G) CDK6 and cyclin D3 expression stained unchanged while CDK2, CDK4 and cyclin E1 expression were down-regulated in THP-1 cells after U73122 treatment