Fig. 1

CD70 Expression Analysis. A mRNA level of CD70 in TCGA sequencing database demonstrated various malignant tissue express higher CD70 compared with normal tissue, especially in KIRC and KIRP, two major types of RCC B CD70 mRNA level in RCC samples according to patient’s clinical stages showed significantly higher expression CD70 associated with later stages (Stage 3 and Stage 4) (n = 72 normal, n = 267 stage 1, n = 57 stage 2, n = 123 stage 3, n = 4 stage 4). C CD70 mRNA level in RCC samples according to pathological grading, showed higher CD70 expression related with higher grades (Grade 3 and Grade 4) (n = 72 normal, n = 14 grade 1, n = 229 grade 2, n = 206 grade 3, n = 76 grade 4). *P < 0.05, **P < 0.01 ***P < 0.001, ****P < 0.0001 by unpaired Student t test. D Lower RCC patients survive related with higher CD70 expression (n = 185 low expression, n = 692 high expression) in TCGA database. E Representative immunohistochemical staining images for CD70 expression in samples from patients with KIRC, KIRP and KICH (Kidney Chromophobe). F Comparison of CD70 expression detected by IHC in samples from 109 RCC patients and 6 normal tissues. Total RCC patients and KIRC, KIRP but not KICH subtypes patients expressed significantly higher CD70 than the normal tissues. *P < 0.05, **P < 0.01 by unpaired Student t test. G Cell surface CD70 expression in RCC cell lines detected by flow cytometry. All RCC cell lines (786-O, 769-P, A498, ACHN) expressed CD70 on cell surface. H Measurement of cell surface CD70 expression level in RCC cell line. Median fluorescence intensity (MFI) ratio calculated by the MFI detected with CD70 antibody divided by antibody isotypes control.