Fig. 5

Mice implanted with brainstem DMG tolerated repeated FUS delivery concurrent with RT. Deacon sequential weightlifting test of (a) the first and (b) the second FUS. Paired motor data for each mouse is represented in different colors for ease of visualization. P-values were calculated by unpaired t tests with Welch’s correction. Body weight curves of RT (black) and RT + FUS (red) animals after (c) the first and (d) the second FUS. Values are means ± SEM. NS indicates non-significant (P > 0.05) in two-way ANOVA. Representative H&E staining of brainstem DMG mice from (e) RT and (f) RT + FUS groups. Each right panel is magnified frm the white square of the left panel. Long arrows indicate cell swelling, vacuolar degeneration, and eosinophilic neurons with pyknosis. Arrow heads indicate mononuclear cell infiltration. Filled star: Blood vessel. Scale bar = 1 mm (left panels), 50 µm (right panels)