Fig. 3
From: MicroRNA-21a-5p inhibition alleviates systemic sclerosis by targeting STAT3 signaling
MiRNA-21a-5p increases the number of STAT3-phosphorylated cells in the skin of bleomycin mice. On days 14, 21, and 28 from the start of bleomycin injection, plasmid containing of pre-miRNA-21a-5p (n = 5) or pre-miR-NC as a control (n = 5) was hydrodynamically injected into the mice. The mice were sacrificed on day 33 from the start of bleomycin injection, and skin tissue sections were stained with antibodies against STAT3, phosphorylated STAT3 at Y705, or S727. The graphs present the number of antibody-positive cells (mean ± SD, n = 5/group). Original magnification: 200 × . Scale bar: 100 µm