Fig. 10

Identification of conserved prenatal diagnostic markers indicating the severity of NTDs. A Assessment of RA-induced NTDs mouse models, exhibiting distinct lesions in the brain or spinal cord. B Relative expression levels of candidate genes for prenatal diagnosis in these mouse models detected by qPCR assay. C Performance of correlation analysis between gene expression (qPCR detected) and the severity of NTDs in mouse models, with R representing the coefficient value. D Application of ROC analysis for prenatal diagnostic genes in the RA-induced mouse model (qPCR detected) and human transcriptome. *P < 0.05; **P < 0.01; ***P < 0.001, non-significant, N.S