Fig. 5

ONCOS-204 induced broad co-stimulation by the soluble production of ICOSL. A Surface ICOS expression on CD4 + (top) and CD8 + (bottom) T cells after 48 h incubation with target cells in different conditions: presence or absence of BsAb and the different A375 tumor cell infections (v: vehicle, ONCOS-102 or ONCOS-204), n = 8. B intracellular ICOS expression on CD4 + (top) and CD8 (bottom) T cells after 48 h incubation with target cells in different conditions. C Surface expression of EGFR (left) and ICOSL (right) on CD4 + (top) and CD8 (bottom) T cells after 48 h incubation with target cells in different conditions (presence of BsAb and ONCOS-204). Quantification of soluble ICOSL concentration in supernatant (in ng/ml, D) and WB of sICOL presence (E) in supernatants 24, 48 and 72 h after infection of A375 with different concentrations of ONCOS-204 (0, 5, 10, 100 and 1000 vp/target cells). F Surface expression of CD69 on ICOSL + and ICOSL- population of CD4 + and CD8 + T cells. G Radar plot of the frequency of CD107a + , IFNγ and TNF + CD4 + and CD8 + T cells after co-culture with A375 tumor cells infected with virus or A375 tumor cells and supernatant issue from virus-infected A375 cells. Student t test or Friedman test with Dunn’s correction was used to compare the two conditions or groups of paired samples exposed to different conditions, B, C, F, G; n = 3. *p < 0.05