Fig. 4

Cytotoxic activity of T cells in combination with EGFRxCD3 BsAb and oncolytic viruses. A Viruses infectivity quantification of A375 tumor cells by RT-qPCR. B Overtime (24 h, 48 h, 4 days) A375 tumor cells viability exposed to 100vp/target cells of vehicle (v), ONCOS-102 or ONCOS-204 virus, assessed by absorbance (490 nm). n = 2 repeats. C A375 tumor cells viability exposed to 100vp/target cells of vehicle, ONCOS-102 or ONCOS-204 virus. Viability assessed 24 h, 48 h and 4 days after PBMCs and BsAb addition. Dot horizontal line corresponds to the medium background absorbance. D specific PBMCs cytotoxic capacity induced by BsAb and vehicle, ONCOS-102 or ONCOS-204 virus. C, D, n = 4. Friedman test with Dunn’s correction was used to compare the groups of paired samples exposed to different conditions. *p < 0.05, **p < 0.01. Medians are represented