Fig. 3

Functional changes induced by EGFRxCD3 BsAb and OVs. A Radar plot of the frequency of CD107a + , IFNγ, TNF + and IL-2 + CD4 + and CD8 + T cells after co-culture with A375 tumor cells infected with different virus. B Frequency of ICOS expression on CD4 + and CD8 + T cells induced by incubation of no or 10 ng/ml EGFRxCD3 bsAb and A375 target cells. C Percentage change of cytokines producing (TNF and IFNγ) and CD107a expressing T cells between CD4 + and CD8 + after co-incubation with tumor cells infected with ONCOS-102 (left) or ONCOS-204 (right). D Differences between change of CD107a expressing and cytokines producing (IL-2, TNF and IFNγ) CD4 + T cells induced by ONCOS-102(top, green) or ONCOS-204 (bottom, red) infected tumor cells. E CD107a, IFNγ and TNF co-expression analysis in CD4 + (top) and CD8 + (bottom) T cells in different conditions: non-infected tumor cells (no virus) vs. tumor cells infected with vehicle or ONCOS-102 or ONCOS-204. Representative histogram of T cells proliferation (F) and proportion of proliferative CD4 + (top) and CD8 + (bottom) T cells (G) after 10 days of incubation with target cells in different conditions: presence BsAb and the different A375 tumor cell infections (v: vehicle, ONCOS-102 or ONCOS-204) and viral concentration (100 vs. 1000 vp/target cells), n = 5. A–E, n = 8. Friedman test with Dunn’s correction was used to compare the groups of paired samples exposed to different conditions. *p < 0.05, **p < 0.01, ***p < 0.001. Medians are represented