Fig. 3

Psoriatic basal keratinocytes as a source of EV miR-625-3p. A and B The expression of miR-625-3p in the skin detected using ISH in A low power field (× 100), and B high-power field (× 400). C Expression levels of miR-625-3p or miR-4488 measured by qRT-PCR after stimulation with 50 ng/ml IL-12 and/or 50 ng/ml IL-23 for 24 h in collected HaCaT or Jurkat cells. D The expression levels of miR-625-3p or miR-4488 in EVs from the cell medium detected by qRT-PCR after stimulation with 50 ng/ml of IL-12 and IL-23 for 24 h. Unpaired Student's t-test was used to compare the two groups. Data are representative of two independent experiments and values are expressed in means ± SEM. *P < 0.05 and **P < 0.01