Fig. 5

Interaction of PinX1 with RBM10 promotes telomerase transport to telomeres. A Knockdown of PinX1 reduced hTERT co-localization with TRF1. B Coomassie brilliant blue staining image of IP assay using anti-PinX1 antibody and IgG in A549 cells. C Mass spectrometry image of RBM10. D A549 cell lines were transfected with a control or shRBM10 lentivirus and validated by western blotting. E–F IP assay and immunofluorescence to detect the content of hTERT bound to coilin in shNC and shPinX1-1 A549 cells. G, H IP assay and immunofluorescence to detect the content of hTERT bound to coilin in shNC and shRBM10-1 A549 cells. I. Knockdown of RBM10 reduced PinX1 co-localization with coilin